SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based)
Isolate pristine Human Classical Monocytes (CD14+CD16-) with the SOLIDEX®-ISOEx Kit. Utilizing a superior Column-based Negative (Untouched) selection strategy, this kit depletes non-target cells from PBMC using optimized Magnetic Beads. The eluted cells are free of antibodies and beads, ensuring Zero Activation and preserving their native phenotype. Achieve High Purity and high viability for sensitive downstream applications like macrophage differentiation.
SOLIDEX®-ISOEx Untouched Human Classical Monocyte Cell Isolation Kit (Column-based) is a Indirect CD14+ Cell Isolation Kit (Column-based, Non-releasable)
주문 정보
| 카탈로그 번호 | 제품명 | 크기 | 가격 (KRW) |
|---|---|---|---|
| GM-Tg-hg-T23212-iso-kit-1-10T | SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) | 10 Tests | KRW 289,750 |
| GM-Tg-hg-T23212-iso-kit-1-50T | SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) | 50 Tests | KRW 869,250 |
| GM-Tg-hg-T23212-iso-kit-1-100T | SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) | 100 Tests | KRW 1,531,100 |
| GM-Tg-hg-T23212-iso-kit-1-750T | SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) | 750 Tests |
사양
- 대상 세포 집단 CD14+CD16- Cell
- 표지 유형 Indirect
- 비드 유형 Non-releasable
- 포맷 Column-based
- 분리 방법 Negative Classical Monocyte Isolation (Untouched)
- 보관 조건 2℃ - 8℃에서 보관
제품 상세
| 카탈로그 번호 | GM-Tg-hg-T23212-iso-kit-1 |
| 제품명 | SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) |
| 영어 이름 | SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit (Column-based) |
| 제품 상세 | SOLIDEX®-ISOEx Untouched Human Classical Monocyte Cell Isolation Kit (Column-based) is a Indirect CD14+ Cell Isolation Kit (Column-based, Non-releasable) |
| 응용 분야 | SOLIDEX®-ISOEx Untouched Human Classical Monocyte Cell Isolation Kit (Column-based) is used for Negative CD14+ Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies |
| 구성품 |
|
| 표지 유형 | Indirect |
| 비드 유형 | Non-releasable |
| 포맷 | Column-based |
| 주요 특징 | Preserves native cell function; maintains surface marker integrity; ideal for functional studies |
| 대상 세포 집단 | CD14+CD16- Cell |
| 보관 조건 | 2℃ - 8℃에서 보관 |
응용 분야
SOLIDEX®-ISOEx Untouched Human Classical Monocyte Cell Isolation Kit (Column-based) is used for Negative CD14+ Cell Isolation (Untouched), with the Key Features, such as Preserves native cell function; maintains surface marker integrity; ideal for functional studies
프로토콜
- Preparation: Prepare a single-cell suspension from cryopreserved or fresh PBMCs.
- Labeling: Add the Biotin-Antibody Cocktail (targets non-CD14 cells: T cells, B cells, NK cells, etc.). Incubate.
- Bead Binding: Add Anti-Biotin Microbeads to bind the labeled non-target cells. Incubate.
- Separation: Apply the suspension to the separation column placed in a magnetic field.
- Elution: Unlabeled CD14+ Classical Monocytes pass through the column freely and are collected (Untouched).
검증 데이터
SOLIDEX®-ISOEx을 사용하여 인간 말초혈액 단핵세포(PBMC)에서 고순도로 고전적 단핵구를 분리 Untouched 인간 고전적 단핵구 분리 키트(컬럼 기반)
카탈로그 번호 GM-Tg-hg-T23212-iso-kit-1
본 실험에서는 SOLIDEX®-ISOEx Untouched 인간 고전적 단핵구 분리 키트(컬럼 기반)를 사용하여 동결보존된 PBMC에서 고전적 단핵구를 분리 및 정제하였습니다. 먼저, 비단핵구 세포를 비오틴 결합 항체 칵테일(항체 칵테일)로 특이적으로 표지한 후, 항비오틴 나노자성 비드와 배양했습니다. 분리 전후의 세포 집단 순도는 유세포 분석으로 분석했습니다. 통과 분획에서 CD14+ 고전적 단핵구의 순도는 21.7%에서 92.1%로 증가했습니다. 실험 결과는 이 키트가 오염 세포를 효과적으로 제거하여 고순도의 손상되지 않은(항체 비결합) 고전적 단핵구를 생산함을 보여줍니다.
그림 14. SOLIDEX®-ISOEx을 사용하여 인간 말초혈액 단핵세포(PBMC)에서 고순도로 고전적 단핵구 분리 Untouched 인간 고전적 단핵구 분리 키트(컬럼 기반).유세포 분석 결과, CD14+ 고전적 단핵구의 순도가 21.7%에서 92.1%로 증가한 것을 보여줍니다.
확대하려면 클릭
배경 정보
Why Choose SOLIDEX®-ISOEx Untouched Human Classical Monocyte Isolation Kit?
- True "Untouched" Isolation: Target monocytes are never bound by antibodies or beads, preventing Fc-receptor activation and preserving differentiation potential.
- Exceptional Purity: The Column-based matrix acts as a physical filter, allowing rigorous washing of contaminants to achieve >90% purity compared to column-free methods.
- Preserved Physiology: Gentle isolation ensures cells remain in a resting state, critical for accurate immunological studies.
- Rapid Workflow: Isolate cells in under 45 minutes with a simple, standardized protocol.
자주 묻는 질문
What are the sample requirements?
We recommend using fresh or cryopreserved Human PBMCs. For cryopreserved samples, ensure high viability (>90%) and proper DNase treatment during thawing to prevent clumping. Leukapheresis products are also compatible.
Can I stain the cells for Flow Cytometry immediately after isolation?
Yes. Since this is an "Untouched" (Negative Selection) kit, the CD14 receptors and other surface markers are free and not blocked by magnetic beads or antibodies. You can proceed directly to staining.
How does the Column-based separation work?
The column contains a ferromagnetic matrix (spheres). When placed in a magnetic field, the matrix amplifies the magnetic force by 10,000-fold, effectively retaining the magnetically labeled non-target cells while unlabeled monocytes pass through.
Are the separation columns reusable?
No. The columns are designed for single-use to ensure sterility and prevent cross-contamination between patient samples.
What is the advantage of Column-based over Column-free methods?
Column-based methods typically offer higher purity. The column allows for washing of the retained cell mass, ensuring that only the specific "Untouched" cells elute. Column-free methods often struggle with non-specific entrapment of target cells in the magnetic clump, leading to lower purity or recovery.
Is this protocol compatible with automated systems?
Yes, the SOLIDEX®-ISOEx column format is compatible with most standard automated magnetic separation instruments designed for column-based protocols.
이 제품에 대한 추가 데이터나 사례가 필요하신가요?
✉ 문의하기 / 견적 요청